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Composition of media used for direct adaptation.
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Composition of media used for direct adaptation.

Journal: International Journal of Molecular Sciences

Article Title: Xeno-Free 3D Bioprinted Liver Model for Hepatotoxicity Assessment

doi: 10.3390/ijms25031811

Figure Lengend Snippet: Composition of media used for direct adaptation.

Article Snippet: In the direct approach, cells were directly cultured in different formulations of DMEM-F12 (L0090, Biowest) with L-glutamine (X0550, Biowest), HEPES (4-(2-Hydroxyethyl)-piperazin-1-ethansulfonsäure, L0180, Biowest), 1× P/S (L0022, Biowest), FCS (S-14-L, c.c.pro GmbH), non-essential amino acids (NEAA, Biowest), GlutaMAX (35050, Gibco, Life Technologies limited, Paisley, UK) Insulin-Transferrin-Selenium (ITS, 41400045, Gibco, Life Technologies Corp, Grand Island, NY, USA), recombinant human hepatocyte growth factor (HGF, PHG0254, Gibco, Life Technologies Corp, Frederick, MD, USA), or recombinant human epidermal growth factor (EGF, PHG0313, Gibco, Life Technologies Corp, Carlsbad, CA, USA).

Techniques: